The Gomori Trichrome is a simplification of the more elaborate Masson stain and combines the plasma stain (chromotrope 2R) with the connective tissue stain to provide a brilliant contrasting picture. Special staining in histopathology - Cite This For Me They can be used to contrast skeletal, cardiac or smooth muscle. Effective image management and automated systems communication are essential for digital pathology success. Store reagents correctly. All our reagents are stored on the shelf above the staining bench. hb```b`` @Q S"40`Fg:yUm;j\r:@]js+&_cUx:&_h6W `: S6p@P6I@h2HXD &-}o 33hqrb!`ZV! Alcian Blue is normally prepared at pH 2.5 and is used to identify acid mucopolysaccharides and acidic mucins. Unsubscribe at any time. tzLGO?h;e+|QL 7{, endstream endobj 36 0 obj <>stream Store reagents correctly. hbbd```b``VA$c)Xd Others have to be left for some time to oxidize (ripen) before they can be used at all. This helps to determine the pattern of tissue injury. California: Do Not Sell or Share My Data. Sometimes we see stray organisms in our sections.. Figure 14: Alcian Blue and PAS (intestine). The latter is utilized to stain peripheral blood smears. There is a tendency for this stain to produce a lot of artefact from background staining, so it is essential to be sure of the morphology of the organism being sought. /s{pEH>A4i,E 5!4EPjl!gBDP 4LJlXuJ b2> PL?qMDkN c`&AYr1Xhq`4f;W"E@k %R,)IEHcH|^r)e.8gPr~ 0 U endstream endobj 39 0 obj <>stream It is used to assist in differentiating collagen and smooth muscle in tumors and assists in the detection of diseases or changes in connective/muscle tissue. Abstract. A primary function of this stain is to identify tuberculosis in lung tissue. The content on this website is intended to be used for informational purposes only. Special stain in histopathology - [PDF Document]
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